| 质粒名称: | pC015 - dLwCas13a-NF |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91905 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGEN |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 5876 bp |
| 完整质粒大小: | 10622 bp |
| 载体类型: | 哺乳细胞表达 |
| 宿生菌： | NEB Stable |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dLwCas13a-eGFP-ZF-KRAB |
| 备用名称： | dead LwCas13a、dead LwC2c2、dCas13a |
| 物种来源: | H. sapiens (human); Leptotrichia wadei |
| 插入大小: | 4806 bp |
| 基因突变: | R474A and R1046A |
| 启动子 | CAG |
| 标签/融合蛋白: |
EGFP（插入件上的C端子）
ZF CCR5TC（插入件上的C端子）
KRAB-A（插入件上的C端子）
NLS（插入件上的C端子）
|
| 5' 测序引物 | pCAG-F |
|
文献引用方法 |
|
| 材料和方法部分: | pC015 - dLwCas13a-NF was a gift from Feng Zhang (Addgene plasmid # 91905 ; http://n2t.net/addgene:91905 ; RRID:Addgene_91905) |
| 参考文献部分: | RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959 |
买家导航
pC015 - dLwCas13a-NF载体质粒基本信息
pC015 - dLwCas13a-NF质粒图谱载体图谱和pC015 - dLwCas13a-NF载体序列质粒序列多克隆位点信息
pC015 - dLwCas13a-NF质粒载体简介
pC015 - dLwCas13a-NF质粒序列载体序列
LOCUS pC015 - dLwCas13a-NF 10715 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses negative-feedback dLwCas13a for mammalian RNA
targeting/imaging.
ACCESSION .
VERSION .
KEYWORDS pC015 - dLwCas13a-NF
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10715)
AUTHORS Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J,
Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES,
Voytas DF, Ting AY, Zhang F
TITLE RNA targeting with CRISPR-Cas13.
JOURNAL Nature. 2017 Oct 4. doi: 10.1038/nature24049.
PUBMED 28976959
REFERENCE 2 (bases 1 to 10715)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10715
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(281..1141)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 904..923
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1142..1246)
/gene="bla"
/label=AmpR promoter
enhancer 1312..1691
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1694..1969
/label=chicken beta-actin promoter
intron 1970..2981
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2905..2927
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2989..3008
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 6561..7271
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELY"
primer_bind complement(6603..6624)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6864..6883)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7211..7232
/label=EGFP-C
/note="EGFP, forward primer"
CDS 7842..7862
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 7879..8011
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 7994..8013
/label=pMT2-F
/note="Synthetic intron, forward primer"
misc_feature 8020..8608
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8073..8093)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8491..8502)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8635..8652)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8641..8752
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(8848..8868)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(8848..8866)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8887..8903)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9009..9028)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 9074..9129
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(9128..9147)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(9490..9506)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 9514..9530
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9538..9568)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9583..9604
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9663..9858
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 9709..9844
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9771..9790
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 9864..9998
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9901..9920)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9955..9974
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10066..10083)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10237..110)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10317..10336)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
61 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
301 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
361 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
421 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
481 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
541 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
601 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
661 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
721 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
781 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
841 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
901 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
961 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1021 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1081 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1141 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1201 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1261 aagtgccacc tgggtcgaca ttgattattg actagagtca tcctcatcgt cgacattgat
1321 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
1381 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
1441 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
1501 gacgtcaatg ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
1561 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
1621 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
1681 ctattaccat gggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
1741 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
1801 gggggggggg gggcgcgcgc caggcggggc ggggcggggg cgagggcggg gcggggcgag
1861 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
1921 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
1981 gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact
2041 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
2101 gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct
2161 ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
2221 tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
2281 ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
2341 tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
2401 cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt
2461 gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc
2521 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
2581 ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg
2641 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
2701 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
2761 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
2821 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
2881 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
2941 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
3001 ttattgtgct gtctcatcat tttggcaaag aattcggtac cgcgggcccg ggcgccacca
3061 tgaaagtgac caaggtcgac ggcatcagcc acaagaagta catcgaagag ggcaagctcg
3121 tgaagtccac cagcgaggaa aaccggacca gcgagagact gagcgagctg ctgagcatcc
3181 ggctggacat ctacatcaag aaccccgaca acgcctccga ggaagagaac cggatcagaa
3241 gagagaacct gaagaagttc tttagcaaca aggtgctgca cctgaaggac agcgtgctgt
3301 atctgaagaa ccggaaagaa aagaacgccg tgcaggacaa gaactatagc gaagaggaca
3361 tcagcgagta cgacctgaaa aacaagaaca gcttctccgt gctgaagaag atcctgctga
3421 acgaggacgt gaactctgag gaactggaaa tctttcggaa ggacgtggaa gccaagctga
3481 acaagatcaa cagcctgaag tacagcttcg aagagaacaa ggccaactac cagaagatca
3541 acgagaacaa cgtggaaaaa gtgggcggca agagcaagcg gaacatcatc tacgactact
3601 acagagagag cgccaagcgc aacgactaca tcaacaacgt gcaggaagcc ttcgacaagc
3661 tgtataagaa agaggatatc gagaaactgt ttttcctgat cgagaacagc aagaagcacg
3721 agaagtacaa gatccgcgag tactatcaca agatcatcgg ccggaagaac gacaaagaga
3781 acttcgccaa gattatctac gaagagatcc agaacgtgaa caacatcaaa gagctgattg
3841 agaagatccc cgacatgtct gagctgaaga aaagccaggt gttctacaag tactacctgg
3901 acaaagagga actgaacgac aagaatatta agtacgcctt ctgccacttc gtggaaatcg
3961 agatgtccca gctgctgaaa aactacgtgt acaagcggct gagcaacatc agcaacgata
4021 agatcaagcg gatcttcgag taccagaatc tgaaaaagct gatcgaaaac aaactgctga
4081 acaagctgga cacctacgtg cggaactgcg gcaagtacaa ctactatctg caagtgggcg
4141 agatcgccac ctccgacttt atcgcccgga accggcagaa cgaggccttc ctgagaaaca
4201 tcatcggcgt gtccagcgtg gcctacttca gcctgaggaa catcctggaa accgagaacg
4261 agaacggtat caccggccgg atgcggggca agaccgtgaa gaacaacaag ggcgaagaga
4321 aatacgtgtc cggcgaggtg gacaagatct acaatgagaa caagcagaac gaagtgaaag
4381 aaaatctgaa gatgttctac agctacgact tcaacatgga caacaagaac gagatcgagg
4441 acttcttcgc caatatcgat gaggccatct ctagtattgc tcacggcatc gtgcatttca
4501 acctggaact ggaaggcaag gacatcttcg ccttcaagaa tatcgccccc agcgagatct
4561 ccaagaagat gtttcagaac gaaatcaacg aaaagaagct gaagctgaaa atcttcaagc
4621 agctgaacag cgccaacgtg ttcaactact acgagaagga tgtgatcatc aagtacctga
4681 agaataccaa gttcaacttc gtgaacaaaa acatcccctt cgtgcccagc ttcaccaagc
4741 tgtacaacaa gattgaggac ctgcggaata ccctgaagtt tttttggagc gtgcccaagg
4801 acaaagaaga gaaggacgcc cagatctacc tgctgaagaa tatctactac ggcgagttcc
4861 tgaacaagtt cgtgaaaaac tccaaggtgt tctttaagat caccaatgaa gtgatcaaga
4921 ttaacaagca gcggaaccag aaaaccggcc actacaagta tcagaagttc gagaacatcg
4981 agaaaaccgt gcccgtggaa tacctggcca tcatccagag cagagagatg atcaacaacc
5041 aggacaaaga ggaaaagaat acctacatcg actttattca gcagattttc ctgaagggct
5101 tcatcgacta cctgaacaag aacaatctga agtatatcga gagcaacaac aacaatgaca
5161 acaacgacat cttctccaag atcaagatca aaaaggataa caaagagaag tacgacaaga
5221 tcctgaagaa ctatgagaag cacaatcgga acaaagaaat ccctcacgag atcaatgagt
5281 tcgtgcgcga gatcaagctg gggaagattc tgaagtacac cgagaatctg aacatgtttt
5341 acctgatcct gaagctgctg aaccacaaag agctgaccaa cctgaagggc agcctggaaa
5401 agtaccagtc cgccaacaaa gaagaaacct tcagcgacga gttggaactg atcaacctgc
5461 tgaacctgga caacaacaga gtgaccgagg acttcgagct ggaagccaac gagatcggca
5521 agttcctgga cttcaacgaa aacaaaatca aggaccggaa agagctgaaa aagttcgaca
5581 ccaacaagat ctatttcgac ggcgagaaca tcatcaagca ccgggccttc tacaatatca
5641 agaaatacgg catgctgaat ctgctggaaa agatcgccga taaggccaag tataagatca
5701 gcctgaaaga actgaaagag tacagcaaca agaagaatga gattgaaaag aactacacca
5761 tgcagcagaa cctgcaccgg aagtacgcca gacccaagaa ggacgaaaag ttcaacgacg
5821 aggactacaa agagtatgag aaggccatcg gcaacatcca gaagtacacc cacctgaaga
5881 acaaggtgga attcaatgag ctgaacctgc tgcagggcct gctgctgaag atcctgcacc
5941 ggctcgtggg ctacaccagc atctgggagc gggacctgag attccggctg aagggcgagt
6001 ttcccgagaa ccactacatc gaggaaattt tcaatttcga caactccaag aatgtgaagt
6061 acaaaagcgg ccagatcgtg gaaaagtata tcaacttcta caaagaactg tacaaggaca
6121 atgtggaaaa gcggagcatc tactccgaca agaaagtgaa gaaactgaag caggagaaga
6181 aagacctgta tattgcaaac tacatcgctc actttaatta tattcctcat gccgagatta
6241 gcctgctgga agtgctggaa aacctgcgga agctgctgtc ctacgaccgg aagctgaaga
6301 acgccatcat gaagtccatc gtggacattc tgaaagaata cggcttcgtg gccaccttca
6361 agatcggcgc tgacaagaag atcgaaatcc agaccctgga atcagagaag atcgtgcacc
6421 tgaagaatct gaagaaaaag aaactgatga ccgaccggaa cagcgaggaa ctgtgcgaac
6481 tcgtgaaagt catgttcgag tacaaggccc tggaaggagg aggtggaagc ggaggaggag
6541 gaagcggagg aggaggtagc gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
6601 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
6661 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
6721 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
6781 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
6841 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
6901 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
6961 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
7021 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
7081 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
7141 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
7201 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
7261 acgagctgta ctctagggga gctggcgctg gagcgggtgc aggggctggc tctagattcc
7321 agtgccggat ctgcatgcgg aacttcagcg accggtccaa cctgagcagg cacatcagaa
7381 cccacaccgg agaaaagccc ttcgcctgcg acatttgcgg ccggaagttc gccatcagca
7441 gcaacctgaa cagccacacc aagatccaca ctggcagcca gaaacctttc cagtgcagaa
7501 tttgtatgag aaactttagc agaagcgaca acctggccag acacatccgg acacatactg
7561 gtgaaaaacc ttttgcctgt gatatctgtg gcagaaagtt tgccacctcc ggcaatctga
7621 cccggcacac aaagattcac ctgcggggca gccagctatc gattgtcgac gctcctgaac
7681 aacgtgaagg tgcttctcaa gtttctgttt ctgttacttt tgaagatgtt gctgttcttt
7741 ttactcgtga tgaatggaaa aaacttgatc tttctcaacg ttctctttat cgtgaagtta
7801 tgcttgaaaa ttattctaat cttgcttcta tggctggatc ccccaagaaa aagcgcaagg
7861 tataactcga ggccgcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag
7921 aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt
7981 actgacatcc actttgcctt tctctccaca ggtgtcgaca atcaacctct ggattacaaa
8041 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
8101 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
8161 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
8221 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
8281 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
8341 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
8401 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
8461 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
8521 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
8581 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacga tcagcctcga
8641 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
8701 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
8761 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
8821 gggaagacaa tagcccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
8881 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctaattcact cctcaggtgc
8941 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
9001 gagatcgatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
9061 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
9121 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
9181 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
9241 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
9301 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
9361 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
9421 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
9481 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
9541 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
9601 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
9661 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
9721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
9781 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
9841 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
9901 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
9961 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
10021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
10081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
10141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
10201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
10261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
10321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
10381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
10441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
10501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
10561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
10621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
10681 agtatttggt atctgcgctc tgctgaagcc agtta
//
